2012
Arias, Dulce M; Albarrán-Lara, Ana L; González-Rodríguez, Antonio; Peñaloza-Ramírez, Juan; Dorado, Oscar; Leyva, Esaú
Genetic diversity and structure of wild populations of the tropical dry forest tree Jacaratia mexicana (Brassicales: Caricaceae) at a local scale in Mexico report
2012.
Resumen | Etiquetas: Bayesian methods, Caricaceae, Genetic diversity, Jacaratia mexicana, population structure, RAPDs
@report{Arias2012,
title = {Genetic diversity and structure of wild populations of the tropical dry forest tree Jacaratia mexicana (Brassicales: Caricaceae) at a local scale in Mexico},
author = {Dulce M Arias and Ana L Albarrán-Lara and Antonio González-Rodríguez and Juan Peñaloza-Ramírez and Oscar Dorado and Esaú Leyva},
year = {2012},
date = {2012-01-01},
journal = {Rev. Biol. Trop. (Int. J. Trop. Biol. ISSN},
volume = {60},
issue = {1},
pages = {1-10},
abstract = {The tropical dry forest is a greatly endangered ecosystem, from which Jacaratia mexicana is a native tree. With the aim to assess the levels of genetic variation and population structure, four wild populations of J. mexicana were studied in the Sierra de Huautla Biosphere Reserve, Morelos, Mexico. For this, DNA was extracted from 159 individuals and were amplified with six random primers using the Random Amplified Polymorphic DNA (RAPD). A total of 54 bands were obtained, of which 50 (92.6%) were polymorphic. The total genetic diversity found within the four populations was 0.451 when estimated by Shannon's index. An AMOvA analysis showed that 84% of the total genetic variation was found within populations and 16% was among populations. The UPGMA dendrogram showed that all individuals from one of the populations (Huaxtla) formed one distinct genetic group, while the rest of the individuals did not cluster according to population. A Mantel test did not show an association between genetic and geographical distances among populations (r=0.893},
keywords = {Bayesian methods, Caricaceae, Genetic diversity, Jacaratia mexicana, population structure, RAPDs},
pubstate = {published},
tppubtype = {report}
}
The tropical dry forest is a greatly endangered ecosystem, from which Jacaratia mexicana is a native tree. With the aim to assess the levels of genetic variation and population structure, four wild populations of J. mexicana were studied in the Sierra de Huautla Biosphere Reserve, Morelos, Mexico. For this, DNA was extracted from 159 individuals and were amplified with six random primers using the Random Amplified Polymorphic DNA (RAPD). A total of 54 bands were obtained, of which 50 (92.6%) were polymorphic. The total genetic diversity found within the four populations was 0.451 when estimated by Shannon's index. An AMOvA analysis showed that 84% of the total genetic variation was found within populations and 16% was among populations. The UPGMA dendrogram showed that all individuals from one of the populations (Huaxtla) formed one distinct genetic group, while the rest of the individuals did not cluster according to population. A Mantel test did not show an association between genetic and geographical distances among populations (r=0.893
2006
Oyama, Ken; Hernández-Verdugo, Sergio; Sánchez, Carla; González-Rodríguez, Antonio; Sánchez-Peña, Pedro; Garzón-Tiznado, José Antonio; Casas, Alejandro
Genetic structure of wild and domesticated populations of Capsicum annuum (Solanaceae) from northwestern Mexico analyzed by RAPDs Artículo de revista
En: Genetic Resources and Crop Evolution, vol. 53, iss. 3, pp. 553-562, 2006, ISSN: 09259864.
Resumen | Enlaces | Etiquetas: capsicum annuum, crop evolution, domestication, Genetic resources conservation, RAPDs, Solanaceae
@article{Oyama2006,
title = {Genetic structure of wild and domesticated populations of Capsicum annuum (Solanaceae) from northwestern Mexico analyzed by RAPDs},
author = {Ken Oyama and Sergio Hernández-Verdugo and Carla Sánchez and Antonio González-Rodríguez and Pedro Sánchez-Peña and José Antonio Garzón-Tiznado and Alejandro Casas},
doi = {10.1007/s10722-004-2363-1},
issn = {09259864},
year = {2006},
date = {2006-01-01},
journal = {Genetic Resources and Crop Evolution},
volume = {53},
issue = {3},
pages = {553-562},
abstract = {Levels of genetic variation and genetic structure of 15 wild populations and three domesticated populations of Capsicum annuum were studied by RAPD markers. A total of 166 bands (all of them polymorphic) and 126 bands (125 of them polymorphic) were amplified in wild and domesticated populations, respectively. Mean percentage of polymorphism was 34.2% in wild populations and 34.7% in domesticated populations. Mean and total genetic diversity were 0.069 and 0.165 for wild populations and 0.081 and 0.131 for domesticated populations. Parameters of genetic diversity estimated from 54 bands with frequencies ≥1 - (3/n) (n = sample size) showed that 56.7% of the total variation was within and 43.3% among wild populations, whereas 67.8% of the variation was within and 32.2% among domesticated populations. AMOVA indicated that total genetic diversity was equally distributed within (48.9 and 50.0%) and among (50.0 and 51.1%) populations in both wild and domesticated samples. Wild and domesticated populations were clearly resolved in a UPGMA dendrogram constructed from Jaccard's distances (average GD = 0.197), as well as by AMOVA (17.2% of variance among populations types},
keywords = {capsicum annuum, crop evolution, domestication, Genetic resources conservation, RAPDs, Solanaceae},
pubstate = {published},
tppubtype = {article}
}
Levels of genetic variation and genetic structure of 15 wild populations and three domesticated populations of Capsicum annuum were studied by RAPD markers. A total of 166 bands (all of them polymorphic) and 126 bands (125 of them polymorphic) were amplified in wild and domesticated populations, respectively. Mean percentage of polymorphism was 34.2% in wild populations and 34.7% in domesticated populations. Mean and total genetic diversity were 0.069 and 0.165 for wild populations and 0.081 and 0.131 for domesticated populations. Parameters of genetic diversity estimated from 54 bands with frequencies ≥1 - (3/n) (n = sample size) showed that 56.7% of the total variation was within and 43.3% among wild populations, whereas 67.8% of the variation was within and 32.2% among domesticated populations. AMOVA indicated that total genetic diversity was equally distributed within (48.9 and 50.0%) and among (50.0 and 51.1%) populations in both wild and domesticated samples. Wild and domesticated populations were clearly resolved in a UPGMA dendrogram constructed from Jaccard's distances (average GD = 0.197), as well as by AMOVA (17.2% of variance among populations types