2022
Ibarra-Laclette, Enrique; Venancio-Rodríguez, Carlos Ariel; Vásquez-Aguilar, Antonio Acini; Alonso-Sánchez, Alexandro G.; Pérez-Torres, Claudia Anahí; Villafán, Emanuel; Ramírez-Barahona, Santiago; Galicia, Sonia; Sosa, Victoria; Rebollar, Eria A.; Lara, Carlos; González-Rodríguez, Antonio; Díaz-Fleisher, Francisco; Ornelas, Juan Francisco
Transcriptional Basis for Haustorium Formation and Host Establishment in Hemiparasitic Psittacanthus schiedeanus Mistletoes Artículo de revista
En: Frontiers in Genetics, vol. 13, 2022, ISSN: 16648021.
Resumen | Enlaces | Etiquetas: haustorium, mistletoe, parasitic plant, Psittacanthus schiedeanus, transcriptome
@article{nokey,
title = {Transcriptional Basis for Haustorium Formation and Host Establishment in Hemiparasitic Psittacanthus schiedeanus Mistletoes},
author = {Enrique Ibarra-Laclette and Carlos Ariel Venancio-Rodríguez and Antonio Acini Vásquez-Aguilar and Alexandro G. Alonso-Sánchez and Claudia Anahí Pérez-Torres and Emanuel Villafán and Santiago Ramírez-Barahona and Sonia Galicia and Victoria Sosa and Eria A. Rebollar and Carlos Lara and Antonio González-Rodríguez and Francisco Díaz-Fleisher and Juan Francisco Ornelas},
doi = {10.3389/fgene.2022.929490},
issn = {16648021},
year = {2022},
date = {2022-01-01},
journal = {Frontiers in Genetics},
volume = {13},
publisher = {Frontiers Media S.A.},
abstract = {The mistletoe Psittacanthus schiedeanus, a keystone species in interaction networks between plants, pollinators, and seed dispersers, infects a wide range of native and non-native tree species of commercial interest. Here, using RNA-seq methodology we assembled the whole circularized quadripartite structure of P. schiedeanus chloroplast genome and described changes in the gene expression of the nuclear genomes across time of experimentally inoculated seeds. Of the 140,467 assembled and annotated uniGenes, 2,000 were identified as differentially expressed (DEGs) and were classified in six distinct clusters according to their expression profiles. DEGs were also classified in enriched functional categories related to synthesis, signaling, homoeostasis, and response to auxin and jasmonic acid. Since many orthologs are involved in lateral or adventitious root formation in other plant species, we propose that in P. schiedeanus (and perhaps in other rootless mistletoe species), these genes participate in haustorium formation by complex regulatory networks here described. Lastly, and according to the structural similarities of P. schiedeanus enzymes with those that are involved in host cell wall degradation in fungi, we suggest that a similar enzymatic arsenal is secreted extracellularly and used by mistletoes species to easily parasitize and break through tissues of the host.},
keywords = {haustorium, mistletoe, parasitic plant, Psittacanthus schiedeanus, transcriptome},
pubstate = {published},
tppubtype = {article}
}
The mistletoe Psittacanthus schiedeanus, a keystone species in interaction networks between plants, pollinators, and seed dispersers, infects a wide range of native and non-native tree species of commercial interest. Here, using RNA-seq methodology we assembled the whole circularized quadripartite structure of P. schiedeanus chloroplast genome and described changes in the gene expression of the nuclear genomes across time of experimentally inoculated seeds. Of the 140,467 assembled and annotated uniGenes, 2,000 were identified as differentially expressed (DEGs) and were classified in six distinct clusters according to their expression profiles. DEGs were also classified in enriched functional categories related to synthesis, signaling, homoeostasis, and response to auxin and jasmonic acid. Since many orthologs are involved in lateral or adventitious root formation in other plant species, we propose that in P. schiedeanus (and perhaps in other rootless mistletoe species), these genes participate in haustorium formation by complex regulatory networks here described. Lastly, and according to the structural similarities of P. schiedeanus enzymes with those that are involved in host cell wall degradation in fungi, we suggest that a similar enzymatic arsenal is secreted extracellularly and used by mistletoes species to easily parasitize and break through tissues of the host.
2011
Cuevas-Reyes, Pablo; Fernandes, G. Wilson; González-Rodríguez, Antonio; Pimenta, Mariana
Effects of generalist and specialist parasitic plants (Loranthaceae) on the fluctuating asymmetry patterns of ruprestrian host plants Artículo de revista
En: Basic and Applied Ecology, vol. 12, iss. 5, pp. 449-455, 2011, ISSN: 16180089.
Resumen | Enlaces | Etiquetas: Fluctuating asymmetry, Host parasite-relationships, mistletoe, Parasitism impact, Rupestrian fields, Serra do Cipó, Serra do Rola Moça
@article{Cuevas-Reyes2011a,
title = {Effects of generalist and specialist parasitic plants (Loranthaceae) on the fluctuating asymmetry patterns of ruprestrian host plants},
author = {Pablo Cuevas-Reyes and G. Wilson Fernandes and Antonio González-Rodríguez and Mariana Pimenta},
doi = {10.1016/j.baae.2011.04.004},
issn = {16180089},
year = {2011},
date = {2011-01-01},
journal = {Basic and Applied Ecology},
volume = {12},
issue = {5},
pages = {449-455},
abstract = {Leaf fluctuating asymmetry (FA) is an important indicator of plant responses to various sources of stress, which range from poor nutritional and harsh environmental conditions to herbivory and parasitism. Mistletoes are important hemiparasites, which represent a source of stress for their host plants but, in spite of being common in all major biogeographical regions of the world, no study has measured the response of their hosts in terms of leaf FA. We addressed the effect of mistletoes on host-plant foliar FA by comparing parasitized and non-parasitized individuals of four host species in two sites in southeastern Brazil, Serra do Cipó and Serra do Rola Moça. In the four host species, parasitized individuals presented significantly higher levels of leaf FA than unattacked plants. The specialist mistletoe Phoradendron cf. amplexicaule induced higher levels of FA on its host Erythroxylum suberosum (Erythroxylaceae) than the generalist mistletoe Struthanthus flexicaulis did on either Mimosa calodendron (Fabaceae), Lychnophora pinaster (Asteraceae) or Stachytarpheta glabra (Verbenaceae). We hypothesized that specialized parasites could be more damaging to their hosts than generalist mistletoes, which could explain the higher FA levels observed. Significant differences in FA levels among parasitized individuals within species were found, which were significantly correlated with intensity of infection (number of mistletoes per host individual). The results indicate that mistletoes represent an important factor of stress for their host plants, and that FA can be an excellent instrument for evaluating the effects of infections by parasitic plants. © 2011 Gesellschaft für Ökologie.},
keywords = {Fluctuating asymmetry, Host parasite-relationships, mistletoe, Parasitism impact, Rupestrian fields, Serra do Cipó, Serra do Rola Moça},
pubstate = {published},
tppubtype = {article}
}
Leaf fluctuating asymmetry (FA) is an important indicator of plant responses to various sources of stress, which range from poor nutritional and harsh environmental conditions to herbivory and parasitism. Mistletoes are important hemiparasites, which represent a source of stress for their host plants but, in spite of being common in all major biogeographical regions of the world, no study has measured the response of their hosts in terms of leaf FA. We addressed the effect of mistletoes on host-plant foliar FA by comparing parasitized and non-parasitized individuals of four host species in two sites in southeastern Brazil, Serra do Cipó and Serra do Rola Moça. In the four host species, parasitized individuals presented significantly higher levels of leaf FA than unattacked plants. The specialist mistletoe Phoradendron cf. amplexicaule induced higher levels of FA on its host Erythroxylum suberosum (Erythroxylaceae) than the generalist mistletoe Struthanthus flexicaulis did on either Mimosa calodendron (Fabaceae), Lychnophora pinaster (Asteraceae) or Stachytarpheta glabra (Verbenaceae). We hypothesized that specialized parasites could be more damaging to their hosts than generalist mistletoes, which could explain the higher FA levels observed. Significant differences in FA levels among parasitized individuals within species were found, which were significantly correlated with intensity of infection (number of mistletoes per host individual). The results indicate that mistletoes represent an important factor of stress for their host plants, and that FA can be an excellent instrument for evaluating the effects of infections by parasitic plants. © 2011 Gesellschaft für Ökologie.